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M94A3262.TXT
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1994-10-25
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Document 3262
DOCN M94A3262
TI The V3 region of HIV-1 isolates prevalent in Taiwan contains a unique
signature pattern in addition to the Thailand E subtype sequence.
DT 9412
AU Chen YM; Sheu TT; Lee CM; Jang YJ; AIDS Research Center, National
Yang-Ming Medical College, Taipei,; Taiwan, R.O.C.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):116 (abstract no. PA0083). Unique
Identifier : AIDSLINE ICA10/94369313
AB OBJECTIVE: To study the genetic variation of the V3 region in the env
gene from HIV-1-infected persons in Taiwan. To conduct phylogenetic
analyses of the Taiwanese HIV-1 isolates. METHODS: HIV-1 proviral DNA
was PCR amplified using nested primers flanking the V3 domain and then
sequenced. The nucleotide sequences and predicted amino acid sequences
were analyzed using PHYLIP program (Phylogeny Inference Package).
RESULTS: Our studies have demonstrated: (i) 18 of 22 (82%) HIV-1
isolates from Taiwan belong to the subtype E, with 91% nucleotide
sequence homology with Thailand E subtype (THA E); (ii) there is an
average of 8% diversity of the V3 loop amino acid sequences in Taiwanese
subtype E HIV-1 (TWN E), and the difference of V3 loop amino acid
sequences between the prototypic TWN E (HIV-1-920027) and THA E strains
is 21%; (iii) in addition to the THA-E sequence as the backbone, the V3
region of TWN E also contains a unique signature pattern; (iv) instead
of having GPGQ at the crown of the V3 loop of the THA E, the TWN E
strains contain GPGR motif; and (v) the amino acid residues 307-309 of
THA E strains V3 loop have changed from SNN to YN in all the TWN E
isolates. DISCUSSIONS AND CONCLUSIONS: The predominant HIV-1 strains
identified in Taiwan during the 1993 and early 1994 period appear to
have evolved from subtype E in Thailand. The limited genetic variation
among different TWN E isolates from persons with different risk factors
also suggests the recent introduction of the subtype E epidemic to
Taiwan.
DE Amino Acid Sequence DNA, Viral/GENETICS Genes, env Human HIV
Envelope Protein gp120/*GENETICS HIV Infections/MICROBIOLOGY
HIV-1/CLASSIFICATION/*GENETICS/*ISOLATION & PURIF Molecular Sequence
Data Peptide Fragments/*GENETICS Phylogeny Polymerase Chain Reaction
Proviruses/GENETICS Sequence Homology, Amino Acid Species Specificity
Taiwan Thailand Variation (Genetics) MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).